Table 2. Probes used for Northern Blot experiments. |
Gene |
Probes |
|
|
|
Template for probe generation |
primers for probe generation |
gene coverage |
CTCF
|
5'- GACTGTCTCTGGACCGCTATCTAA and 3'- TTGAAGTTTTCGTTCTCGGTATGT primers were used on BAC clone RP11-167P11, and the PCR product was inserted into pCR2.1-TOPO (Invitrogen) |
M13 (-20) Forward primer
M13 Reverse Primer |
exon 12- 3' UTR |
E2F4
|
IMAGE EST clone ID 786048 (Unigene Cluster Hs.108371) vector pT3T7-Pac |
M13 (-20) Forward primer
M13 Reverse Primer |
exon 8-3'UTR |
NFATC3
|
IMAGE EST clone ID 727192 (Unigene Cluster Hs.172674) vector pT7T3D-Pac |
M13 (-20) Forward primer
M13 Reverse Primer |
exon 9-3'UTR |
NFAT5
|
IMAGE EST clone ID 753973 (Unigene Cluster Hs.86998) vector pT3T7-Pac |
M13 (-20) Forward primer
M13 Reverse Primer |
exon 14 |